goat polyclonal anti substance p  (Santa Cruz Biotechnology)

Journal: PloS one

Article Title: Vertical transmission of respiratory syncytial virus modulates pre- and postnatal innervation and reactivity of rat airways.

doi: 10.1371/journal.pone.0061309

Figure Lengend Snippet: Figure 4. Propagation of vertically transmitted RSV. Extracts of whole fetuses delivered from dams inoculated with rrRSV or from pathogen- free controls and co-cultured with human airway epithelial cells. After 48 h of incubation, the cells were then stained with a polyclonal anti-NGF antibody. The micrographs show red fluorescence in the cytoplasm of cells exposed to fetal extracts from rrRSV-infected dams, confirming the presence of actively replicating infectious virus that is associated with markedly increased green NGF immunoreactivity. As RFP expression reflects virus infection and production of the transgene rather than virus spread and plaque formation, this methodology is more accurate than the standard plaque assay to assess RSV infectivity. Magnification = 60X. doi:10.1371/journal.pone.0061309.g004

Article Snippet: To localize adrenergic, cholinergic, and non-adrenergic noncholinergic (NANC) nerves in fetal tissues, frozen sections were fixed in cold acetone for 10 min at 4uC, washed 3 times with PBS, and stained for 1 h with one of the following primary antibodies: a. polyclonal rabbit anti- noradrenaline (Abcam); b. polyclonal rabbit anti-acetylcholine (AbD Serotec, Raleigh, NC); or c. polyclonal goat anti-substance P (Santa Cruz Biotechnology).

Techniques: Cell Culture, Incubation, Staining, Fluorescence, Infection, Virus, Expressing, Plaque Assay

Journal: PloS one

Article Title: Vertical transmission of respiratory syncytial virus modulates pre- and postnatal innervation and reactivity of rat airways.

doi: 10.1371/journal.pone.0061309

Figure Lengend Snippet: Figure 5. Localization of vertically transmitted RSV. Sections of fetuses delivered from dams inoculated with rrRSV or from pathogen-free controls co-stained with polyclonal antibodies against RFP and NGF. Immunohistochemical analysis of fetal airways (asterisks) by confocal microscopy localized increased green NGF immunoreactivity to the epithelial layer, overlapping areas of yellow RSV immunoreactivity (arrows). No significant fluorescence was detected in any other fetal structures. Magnification = 60X. doi:10.1371/journal.pone.0061309.g005

Article Snippet: To localize adrenergic, cholinergic, and non-adrenergic noncholinergic (NANC) nerves in fetal tissues, frozen sections were fixed in cold acetone for 10 min at 4uC, washed 3 times with PBS, and stained for 1 h with one of the following primary antibodies: a. polyclonal rabbit anti- noradrenaline (Abcam); b. polyclonal rabbit anti-acetylcholine (AbD Serotec, Raleigh, NC); or c. polyclonal goat anti-substance P (Santa Cruz Biotechnology).

Techniques: Staining, Immunohistochemical staining, Confocal Microscopy, Fluorescence

Journal: PloS one

Article Title: Vertical transmission of respiratory syncytial virus modulates pre- and postnatal innervation and reactivity of rat airways.

doi: 10.1371/journal.pone.0061309

Figure Lengend Snippet: Figure 6. Cholinergic innervation of RSV-infected fetal airways. Sections of fetuses delivered from dams inoculated with rrRSV or from pathogen-free controls co-stained with polyclonal antibodies against acetylcholine and NGF. Immunohistochemical analysis by confocal microscopy found increased acetylcholine immunoreactivity in the subepithelial neural plexus lining the lumen of fetal airways exposed in utero to RSV and co- expressing NGF immunoreactivity (asterisks). In contrast, noradrenaline and substance P immunoreactivity was very weak in all fetuses. doi:10.1371/journal.pone.0061309.g006

Article Snippet: To localize adrenergic, cholinergic, and non-adrenergic noncholinergic (NANC) nerves in fetal tissues, frozen sections were fixed in cold acetone for 10 min at 4uC, washed 3 times with PBS, and stained for 1 h with one of the following primary antibodies: a. polyclonal rabbit anti- noradrenaline (Abcam); b. polyclonal rabbit anti-acetylcholine (AbD Serotec, Raleigh, NC); or c. polyclonal goat anti-substance P (Santa Cruz Biotechnology).

Techniques: Infection, Staining, Immunohistochemical staining, Confocal Microscopy, In Utero, Expressing